Dose : 1 ml by intramuscular injection in breast or thigh muscle per chicken. However, the following symptoms should be observed five days-incubation period-before 8th week :
a] Chickens showing severe depression and developing respiratory distress.
b] Chickens having drooping wings and closing their eyes most of the times.
c] The chickens passing greenish yellow diarrheas
d] Chickens with combs and wattles which are pale and shrunken respectively.
The chickens exhibiting the above should not be vaccinated for symptoms of fowl typhoid.